Survey Role of Surface Structure Layer and Enzyme in Bacterial Antibiotics Resistant (AZZAHRA Hospital–Isfahan-Iran)
Shilla Jalalpoor Teacher of Microbiology,
Islamic Azad University Shahreza Branch,
Membership of Young Researchers Club.
Islamic Republic of Iran
Abstract:
Background and Objectives:
S-layer is outermost protein in bacteria and archiae and with antibiotics introduce inhibit due to increase pathogenicity in bacteria, according role of staff hands and hospital surfaces in nosocomial infections, therefore contamination this source whit B.cereus strain that have S-layer and β–lactamase, due to spread antibiotic resistant nosocomial infections ,objective this search were survey frequency S-layer and β–lactamase so role S-layer in antibiotics introduce inhibit in B.cereus strain isolated of staff hands and hospital surfaces.
Material and Methods:
The research were performed with laboratory method and performed in 2005/2007 years, in Azzahra hospital and Isfahan University andStudy 274 sample, for preparation samples, culture bacteria in TSA (Tryptone Soya Agar), for 16h,then separated surface proteins and, specimen's electrophoresis with 10X SDS-PAGE.Samples antibiogram were performed with Kirby Bauer method and β–lactamase production, detection with acidimetric method.
Results:
From 13 isolated B.cereus of, staff hand 11 sample (84/6%) and from 13 isolated B.cereus from hospital surfaces, 1 sample (7/7%) production nano structure S-layer. According to antibiogram result, strain without S-layer, in comparative strain with S-layer, were sensitive to antibiotics and all B.cereus strain that have S-layer, production β–lactamase.
Conclusion:
Result show prevalence B.cereus strain with S-layer and β–lactamase in hospital sensitive environment, that due to increase antibiotic resistance nosocomial infection and is necessary go on to reduce transfer virulence agent and antibiotic resistant in pathogen bacteria.
